This email arrived in my inbox this afternoon, right around lunch time:
I am about to start working on a pilot study in which we will attempt to digest a whole mouse brain and sort the neurons on a FACSCalibur (because we don't have a high speed sorter yet). Can anyone offer me some protocol advice on how to go about digesting the brain without it disintegrating completely and/or point me to some groups that do this
sort of thing? I hear there is a group in Australia that works with sorting neurons, but I don't know where/who.
This email makes perfect sense to me. However, when I stop for a minute and take it out of professional context it's pretty hilarious.